| Gene | Nek3 |
| Chromosome | 8 |
| Deletion Start | 22,162,437 |
| Deletion End | 22,128,592 |
| Deletion Size | 33,846 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,162,414 | 22,162,437 | GACAACTACACAGTCCTGAGAGTG |
| TUP | 22,162,393 | 22,162,412 | TTGGCCAGGGCTCCTTCGGC |
| TUR | 22,162,316 | 22,162,334 | GCCGTTACCTTGAGCAGTC |
| TDF | 22,129,476 | 22,129,497 | CCACACTTTCTGTTGCTGTTCC |
| TDP | 22,129,447 | 22,129,475 | CCTGAAGGTGCAGAAGGATTCCTGAAAGG |
| TDR | 22,129,417 | 22,129,436 | CTGTCCGATGCCGTGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TTTAAGGCCCTGGCAGAC |
| SD | GAAAGGCAGCTGGTAGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17871A-H6 | VGB6 | C57BL/6NTac | |
| 17871A-E3 | VGB6 | C57BL/6NTac | |
| 17871A-A3 | VGB6 | C57BL/6NTac | |
| 17871A-G2 | VGB6 | C57BL/6NTac | |
| 17871A-C2 | VGB6 | C57BL/6NTac | |
| 17871A-D3 | VGB6 | C57BL/6NTac | |
