| Gene | Nup50 |
| Chromosome | 15 |
| Deletion Start | 84,939,867 |
| Deletion End | 84,927,381 |
| Deletion Size | 12,487 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,927,413 | 84,927,434 | CAGGAACTGGGATGAGGAAGAC |
| TUP | 84,927,437 | 84,927,462 | AGTTGAAGAGGTACAGAGCCGCTCCC |
| TUR | 84,927,468 | 84,927,489 | GCTGAGACACAGACAGCAAGAG |
| TDF | 84,938,973 | 84,938,993 | TCTTACGTGGGTTCTGAGGAC |
| TDP | 84,938,997 | 84,939,023 | AAGTCAGGTCCTTGTGCTTGCATGCTG |
| TDR | 84,939,053 | 84,939,075 | CTCTGGCTCTATGGAAAGCACTC |
PCR Primers
| Primer | Sequence |
| SU | TGCTCTGTAGCCCTGTGTTG |
| SD | GCCTTTGGAGAGCTAACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17872A-G4 | VGB6 | C57BL/6NTac | |
| 17872A-E12 | VGB6 | C57BL/6NTac | |
| 17872A-F9 | VGB6 | C57BL/6NTac | |
| 17872A-F3 | VGB6 | C57BL/6NTac | |
| 17872A-D12 | VGB6 | C57BL/6NTac | |
