| Gene | Rundc1 |
| Chromosome | 11 |
| Deletion Start | 101,434,340 |
| Deletion End | 101,425,107 |
| Deletion Size | 9,234 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,425,601 | 101,425,621 | TGAGGACCAGGTGAGTGTCTG |
| TUP | 101,425,622 | 101,425,644 | CCGCTAGTTCCGGATGCGGATGA |
| TUR | 101,425,663 | 101,425,682 | CAGCATCACCACCCACAAAC |
| TDF | 101,434,018 | 101,434,038 | AGCCTTCTGACAGCCATCCAC |
| TDP | 101,434,040 | 101,434,061 | TGGTGCTGACAGAGCATGACCC |
| TDR | 101,434,089 | 101,434,108 | CCATGCACACCAACGCCTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGGACTCCAGAAGGACACC |
| SD | ACCGAGCTACACCTCTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17875A-C2 | VGB6 | C57BL/6NTac | |
| 17875A-C4 | VGB6 | C57BL/6NTac | |
| 17875A-E12 | VGB6 | C57BL/6NTac | |
