| Gene | Sgms1 |
| Chromosome | 19 |
| Deletion Start | 32,160,161 |
| Deletion End | 32,124,340 |
| Deletion Size | 35,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,159,873 | 32,159,894 | CTCAGCATTGGCGTTGACATTC |
| TUP | 32,159,845 | 32,159,867 | CCCGATGGCAGCTTCAGCATCAA |
| TUR | 32,159,824 | 32,159,844 | GGCATTCCGTTGGGTTTAGTC |
| TDF | 32,125,192 | 32,125,216 | CCCTTCCCTGTCATCTTAACTTCTC |
| TDP | 32,125,166 | 32,125,188 | TCTCAGTCTTGGTGCTGCTGGCT |
| TDR | 32,125,135 | 32,125,158 | CCGTCTTTCCAACACCTCTTACTC |
PCR Primers
| Primer | Sequence |
| SU | CTGTTACCCGAGGAAGATGG |
| SD | TCCAGATATGGCTGGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17876A-A3 | VGB6 | C57BL/6NTac | |
| 17876A-A11 | VGB6 | C57BL/6NTac | |
| 17876A-B9 | VGB6 | C57BL/6NTac | |
| 17876A-G8 | VGB6 | C57BL/6NTac | |
| 17876A-F7 | VGB6 | C57BL/6NTac | |
