| Gene | Stap2 |
| Chromosome | 17 |
| Deletion Start | 56,005,528 |
| Deletion End | 55,997,282 |
| Deletion Size | 8,247 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,005,283 | 56,005,303 | CACCTAGTGGAGCGAGCATAC |
| TUP | 56,005,256 | 56,005,281 | CCACTGGCTGTGGAAGGTTTGGCCAT |
| TUR | 56,005,206 | 56,005,228 | CAGGAAGGCAGACAGATAGACAG |
| TDF | 55,997,540 | 55,997,559 | GGACCTCATCTGCCTCTTTC |
| TDP | 55,997,513 | 55,997,539 | TGCAGATCTCAAAGCCATTACCAGCGT |
| TDR | 55,997,486 | 55,997,506 | GAGCTTCCACCTAGCTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GGTGTCAGCAAAGTGGGTTC |
| SD | CTGCTGCTTGGAGCCTTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17877A-E12 | VGB6 | C57BL/6NTac | |
| 17877A-G4 | VGB6 | C57BL/6NTac | |
| 17877A-F6 | VGB6 | C57BL/6NTac | |
| 17877A-H6 | VGB6 | C57BL/6NTac | |
| 17877A-D6 | VGB6 | C57BL/6NTac | |
