| Gene | Sun1 |
| Chromosome | 5 |
| Deletion Start | 139,248,611 |
| Deletion End | 139,215,181 |
| Deletion Size | 33,431 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,215,996 | 139,216,017 | CACCATGTTTGGTTGGTCAGTC |
| TUP | 139,216,019 | 139,216,041 | AGCTAGCCGGTTTGTTCCTGGGA |
| TUR | 139,216,052 | 139,216,072 | GTCCATAGTCCCAGTGCTCAG |
| TDF | 139,248,424 | 139,248,445 | CCTGACCAAGCCTTCCAGATAG |
| TDP | 139,248,446 | 139,248,468 | TAGAGCTCCGGGTCCTGTCCAAC |
| TDR | 139,248,506 | 139,248,524 | ATGGGCTCTCCGTGGACTC |
PCR Primers
| Primer | Sequence |
| SU | ACAAGCATGGAGGTCAGAGG |
| SD | AGTCCCAGCTCAAAGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17878A-E3 | VGB6 | C57BL/6NTac | |
| 17878A-F9 | VGB6 | C57BL/6NTac | |
| 17878A-H8 | VGB6 | C57BL/6NTac | |
| 17878A-E12 | VGB6 | C57BL/6NTac | |
