| Gene | Surf4 |
| Chromosome | 2 |
| Deletion Start | 26,933,319 |
| Deletion End | 26,921,805 |
| Deletion Size | 11,515 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,932,999 | 26,933,019 | GTCCTGGCCCTTCCTTCTTTG |
| TUP | 26,932,977 | 26,932,998 | CGTGGACTCTGCTCCGGTTGCC |
| TUR | 26,932,888 | 26,932,907 | CCTTCCCTGCGGTTCACAAG |
| TDF | 26,922,091 | 26,922,109 | GCTGGCAGCTTTGACTCTC |
| TDP | 26,922,066 | 26,922,089 | TCGTCTGGCTGTTTGCCATCAACG |
| TDR | 26,922,043 | 26,922,064 | TGTCCAGAAGGCGTTGAAATAC |
PCR Primers
| Primer | Sequence |
| SU | TTTAAGGCTTGGCCTACGTG |
| SD | CTGGAGGTTTAACCGTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17879A-A5 | VGB6 | C57BL/6NTac | |
| 17879A-B3 | VGB6 | C57BL/6NTac | |
| 17879A-C2 | VGB6 | C57BL/6NTac | |
| 17879A-B11 | VGB6 | C57BL/6NTac | |
