| Gene | Ttll3 |
| Chromosome | 6 |
| Deletion Start | 113,414,340 |
| Deletion End | 113,397,843 |
| Deletion Size | 16,498 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,398,556 | 113,398,578 | GCTGACGTGTTAGGAAGGTTGAG |
| TUP | 113,398,579 | 113,398,608 | AGCCACTGAACTATAAAGGCTTCCTGGGTA |
| TUR | 113,398,611 | 113,398,630 | ACCCACTCTTTCCCATCCAG |
| TDF | 113,414,029 | 113,414,048 | GATGTCCTTCCCACCTCACC |
| TDP | 113,414,053 | 113,414,075 | TCTCAAGCTGGCACCTAGCATGC |
| TDR | 113,414,082 | 113,414,100 | TCGAAGCCCACCTTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | AAGGAGGAAGGATGGAAAGG |
| SD | TTGTGGGCCTTTGATAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17880A-G12 | VGB6 | C57BL/6NTac | |
| 17880A-A12 | VGB6 | C57BL/6NTac | |
| 17880A-B6 | VGB6 | C57BL/6NTac | |
| 17880A-E9 | VGB6 | C57BL/6NTac | |
| 17880A-E12 | VGB6 | C57BL/6NTac | |
| 17880A-D9 | VGB6 | C57BL/6NTac | |
