| Gene | Xrcc1 |
| Chromosome | 7 |
| Deletion Start | 24,573,363 |
| Deletion End | 24,547,312 |
| Deletion Size | 26,052 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,547,760 | 24,547,782 | GAACTTCCACCTTTCCACCCTTG |
| TUP | 24,547,783 | 24,547,805 | CCTGCAGACCCATTGTGCCGAAA |
| TUR | 24,547,811 | 24,547,830 | CGGTAAGTGTCCGCCTTGAG |
| TDF | 24,573,070 | 24,573,090 | ATGAATGAGCGGGTCCAGTTC |
| TDP | 24,573,093 | 24,573,114 | CATCACGGCCCAGGAGTGGGAC |
| TDR | 24,573,120 | 24,573,139 | CAGGGCTCACCTCCTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATGCCTGTTGCTAAGGGAAC |
| SD | AAGAACAGCCTTCCTCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17881A-D10 | VGB6 | C57BL/6NTac | |
| 17881A-F4 | VGB6 | C57BL/6NTac | |
| 17881A-G2 | VGB6 | C57BL/6NTac | |
| 17881A-G8 | VGB6 | C57BL/6NTac | |
| 17881A-H6 | VGB6 | C57BL/6NTac | |
| 17881A-B3 | VGB6 | C57BL/6NTac | |
