| Gene | Phf6 |
| Chromosome | X |
| Deletion Start | 52,953,596 |
| Deletion End | 52,916,602 |
| Deletion Size | 36,995 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,916,669 | 52,916,692 | GAGACAAGGAATGTGGACAGTTAC |
| TUP | 52,916,698 | 52,916,723 | TCTGAAAACCAGAAGGTGGCAGCACA |
| TUR | 52,916,724 | 52,916,742 | ACTCACCATGCACTTGTGG |
| TDF | 52,953,243 | 52,953,264 | CAAGTTCTGGGTGTCTGTTGTC |
| TDP | 52,953,265 | 52,953,292 | TCCTTCTGACAATAAGCATGTGGGCCTA |
| TDR | 52,953,359 | 52,953,378 | AGCAGAGGATGTTGGGTCTC |
PCR Primers
| Primer | Sequence |
| SU | GTCCTCAGGCTCACCTTGTG |
| SD | CTCTCTCTGTCCCACGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17883A-C1 | VGB6 | C57BL/6NTac | |
| 17883A-E1 | VGB6 | C57BL/6NTac | |
| 17883A-E12 | VGB6 | C57BL/6NTac | |
| 17883A-G4 | VGB6 | C57BL/6NTac | |
