17887   Dcaf7     Status: ES cell colonies screened / QC positives

GeneDcaf7
Chromosome11
Deletion Start106,054,886
Deletion End106,037,127
Deletion Size17,760
Genome Build38
MGI IDMGI:1919083
NCBI Gene ID71833
BAC ID396P9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,037,616106,037,635AAGATGCGGCGCTTGGATTG
TUP106,037,637106,037,658TGGTCGTGCATTCCTGGCCACG
TUR106,037,708106,037,730AGGGCTCTGTATCCATGAGAAAC
TDF106,054,447106,054,470GAGCTTAATGACACCTGGGAAATG
TDP106,054,480106,054,509CCTAGATTTGGTCCTCACTGGCTACATGGC
TDR106,054,512106,054,531GTCACCAGACTTGCTCCTTC

PCR Primers

PrimerSequence
SUAACAGTTTGGAAAGGACGTAC
SDCCTCAGCAGCAGACAGACAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR115 bp++-
TDFTDR85 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17887A-C2VGB6C57BL/6NTac
17887A-C3VGB6C57BL/6NTac
17887A-F3VGB6C57BL/6NTac
17887A-G2VGB6C57BL/6NTac
17887A-G6VGB6C57BL/6NTac
17887A-A1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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