| Gene | Far1 |
| Chromosome | 7 |
| Deletion Start | 113,568,359 |
| Deletion End | 113,539,281 |
| Deletion Size | 29,079 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,539,419 | 113,539,437 | GCTGGTCAGACACCCCAAG |
| TUP | 113,539,438 | 113,539,464 | AGCGAGTGGAGGAAATCCTCAGTAGCA |
| TUR | 113,539,467 | 113,539,487 | ACGCTCAGCTCCCAAACTTAC |
| TDF | 113,567,666 | 113,567,687 | TTCTGTGGCCTAGTCTGACCTC |
| TDP | 113,567,692 | 113,567,717 | TCAGATCTTCCTGCCTAGGTTCAGCC |
| TDR | 113,567,739 | 113,567,758 | AGCCAAGCCTGGCCTCATTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGAGTGAGTTTGCTGACATC |
| SD | TCTAGGCCTTCTGTCTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17889A-B6 | VGB6 | C57BL/6NTac | |
| 17889A-H6 | VGB6 | C57BL/6NTac | |
| 17889A-E6 | VGB6 | C57BL/6NTac | |
| 17889A-F10 | VGB6 | C57BL/6NTac | |
| 17889A-F8 | VGB6 | C57BL/6NTac | |
| 17889A-B3 | VGB6 | C57BL/6NTac | |
