| Gene | Gm711 |
| Chromosome | 2 |
| Deletion Start | 26,953,474 |
| Deletion End | 26,935,326 |
| Deletion Size | 18,149 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,936,037 | 26,936,060 | CCACAAGAGTACCCTAGAAAGGAG |
| TUP | 26,936,064 | 26,936,090 | TGCTATACTAGTCCCTGTGCACCTTCC |
| TUR | 26,936,095 | 26,936,114 | CAAGGTCCCTGTCAGCACTG |
| TDF | 26,953,093 | 26,953,112 | GCTCCACATGATGCCTTTCC |
| TDP | 26,953,116 | 26,953,143 | TGACTGGGCTGAATCCAATCCCAAATGG |
| TDR | 26,953,144 | 26,953,163 | AGGTGGTCTGGCTGCACTTC |
PCR Primers
| Primer | Sequence |
| SU | TGGCGAAGGGTACTAGATGG |
| SD | TTCTCCAGAGAGTCCTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17892A-A11 | VGB6 | C57BL/6NTac | |
| 17892A-B2 | VGB6 | C57BL/6NTac | |
