| Gene | Pdcd2 |
| Chromosome | 17 |
| Deletion Start | 15,527,270 |
| Deletion End | 15,520,347 |
| Deletion Size | 6,924 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,526,417 | 15,526,436 | TGCAGCAAGGAGCATCAGAC |
| TUP | 15,526,393 | 15,526,415 | TTAGACTGGCGGCTGGGACACAA |
| TUR | 15,526,342 | 15,526,363 | TGACAGAGACGGTAAGGCTAAG |
| TDF | 15,520,819 | 15,520,838 | TCTTCCGAAGGACCCAAGTC |
| TDP | 15,520,789 | 15,520,814 | TCCTAGCATTCACATCAGGAAGCGCA |
| TDR | 15,520,760 | 15,520,781 | CCTCTGGAGCTGGCTATTACAG |
PCR Primers
| Primer | Sequence |
| SU | GAAAGGCTACAGCTGCGAAC |
| SD | GGTAGTAGAGTGTGATGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17894A-B11 | VGB6 | C57BL/6NTac | |
| 17894A-B12 | VGB6 | C57BL/6NTac | |
| 17894A-G8 | VGB6 | C57BL/6NTac | |
| 17894A-E8 | VGB6 | C57BL/6NTac | |
| 17894A-E4 | VGB6 | C57BL/6NTac | |
