| Gene | Rab5c |
| Chromosome | 11 |
| Deletion Start | 100,720,077 |
| Deletion End | 100,715,981 |
| Deletion Size | 4,097 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,719,957 | 100,719,975 | AGCCTGGTCCTCCGCTTTG |
| TUP | 100,719,922 | 100,719,948 | CAGTTCCATGAGTACCAGGAGAGCACA |
| TUR | 100,719,885 | 100,719,905 | CCTCTGGCCTGACTTGCTTTG |
| TDF | 100,716,340 | 100,716,359 | GCCTCAGGAATCCTTCTGTC |
| TDP | 100,716,312 | 100,716,337 | TGAGTTCAAATCCTGGCAATGCCGGT |
| TDR | 100,716,263 | 100,716,285 | TGTGTCCCTAGAAACTGAAGCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAAGCCACTTTGCTTGG |
| SD | CCTAAGACGCTCCCAACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17896A-B4 | VGB6 | C57BL/6NTac | |
| 17896A-B10 | VGB6 | C57BL/6NTac | |
| 17896A-E6 | VGB6 | C57BL/6NTac | |
| 17896A-E11 | VGB6 | C57BL/6NTac | |
| 17896A-C8 | VGB6 | C57BL/6NTac | |
