| Gene | Tead3 |
| Chromosome | 17 |
| Deletion Start | 28,350,592 |
| Deletion End | 28,332,690 |
| Deletion Size | 17,903 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,349,968 | 28,349,987 | ATTGTGGGCGACAGGGCTTC |
| TUP | 28,349,944 | 28,349,967 | CCTTCCCACTCGGGTCCTCTCCAT |
| TUR | 28,349,911 | 28,349,933 | CCTTCCCAGGCAGGAATAAAGAC |
| TDF | 28,333,244 | 28,333,263 | GCCGACAGCATGACCATCAG |
| TDP | 28,333,217 | 28,333,241 | TCTCCACCAAGGTCTGCTCCTTCGG |
| TDR | 28,333,130 | 28,333,149 | GACTGCGTGTCGTGTCATTG |
PCR Primers
| Primer | Sequence |
| SU | GGGAACTCTGAGACGACGAG |
| SD | AGCAACCACACACTAAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 134 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17898A-G4 | VGB6 | C57BL/6NTac | |
| 17898A-F6 | VGB6 | C57BL/6NTac | |
| 17898A-B6 | VGB6 | C57BL/6NTac | |
| 17898A-E7 | VGB6 | C57BL/6NTac | |
| 17898A-A3 | VGB6 | C57BL/6NTac | |
| 17898A-E9 | VGB6 | C57BL/6NTac | |
