| Gene | Top3a |
| Chromosome | 11 |
| Deletion Start | 60,777,098 |
| Deletion End | 60,740,455 |
| Deletion Size | 36,644 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,776,603 | 60,776,627 | CCTTGGTCAATCACAAGTTCAGTAG |
| TUP | 60,776,567 | 60,776,596 | AAAGCGGTAACCAACTCCTTTATCTTGGCG |
| TUR | 60,776,535 | 60,776,559 | GCAGGGACAGGATTCCATAAGTAAG |
| TDF | 60,741,182 | 60,741,200 | AGAAAGCAGGGCTGGAGAG |
| TDP | 60,741,153 | 60,741,180 | TGGCTCAGTTACTAAGAACACTTGCTGC |
| TDR | 60,741,117 | 60,741,134 | TGGGTGCTGGAACTGAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGAGGCATTCTGGGAGTTG |
| SD | CTAGCCACCTCTCCAACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17899A-E9 | VGB6 | C57BL/6NTac | |
| 17899B-B9 | VGB6 | C57BL/6NTac | |
| 17899B-C3 | VGB6 | C57BL/6NTac | |
| 17899B-C9 | VGB6 | C57BL/6NTac | |
