17900   Ttll4     Status: ES cell colonies screened / QC positives

GeneTtll4
Chromosome1
Deletion Start74,697,715
Deletion End74,678,995
Deletion Size18,721
Genome Build38
MGI IDMGI:1914784
NCBI Gene ID67534
BAC ID341H2
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,679,16274,679,180GAAGCACGTAGGCACACTG
TUP74,679,18374,679,205AGCAGGGCTGGGCACCAGTTTCT
TUR74,679,21574,679,237AAGGAAATAGGCTGGCTGGGATG
TDF74,697,10574,697,123CGAGTCCCAAGACCCTGAG
TDP74,697,12474,697,147TGTTCCTTCCTGAACTCAGCCTGC
TDR74,697,18174,697,200TGAGCGATGTTGGCAGAGAC

PCR Primers

PrimerSequence
SUGCAGGATGTCTCCTGATCC
SDAAATCACACATGGCTCTCAGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR76 bp++-
TDFTDR96 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17900A-A11VGB6C57BL/6NTac
17900A-C4VGB6C57BL/6NTac
17900A-D3VGB6C57BL/6NTac
17900A-E5VGB6C57BL/6NTac
17900A-H4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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