| Gene | Vat1 |
| Chromosome | 11 |
| Deletion Start | 101,466,130 |
| Deletion End | 101,460,153 |
| Deletion Size | 5,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,465,616 | 101,465,638 | CGGGTGGACTAAACATGGAACAC |
| TUP | 101,465,582 | 101,465,609 | AGCAATTTATGTCTCCAGGCGTCTGGGA |
| TUR | 101,465,558 | 101,465,578 | TTGCCAGTTCCCAGTAAATCC |
| TDF | 101,460,473 | 101,460,491 | GGGCTACCTGGATGGAGAG |
| TDP | 101,460,446 | 101,460,471 | TGGAACTCGTCAATAGCGTGGTCACC |
| TDR | 101,460,408 | 101,460,428 | GGTTTGATGTGGCCCTGATTG |
PCR Primers
| Primer | Sequence |
| SU | ACCTGAGGCACAATTTCACC |
| SD | AAGCACACAGGATAGGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17901A-B11 | VGB6 | C57BL/6NTac | |
| 17901A-D3 | VGB6 | C57BL/6NTac | |
| 17901A-D11 | VGB6 | C57BL/6NTac | |
| 17901A-G1 | VGB6 | C57BL/6NTac | |
| 17901A-G10 | VGB6 | C57BL/6NTac | |
