| Gene | Yipf5 |
| Chromosome | 18 |
| Deletion Start | 40,218,852 |
| Deletion End | 40,206,141 |
| Deletion Size | 12,712 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,218,068 | 40,218,087 | AGTGGTCGCTCCCTAGAGTG |
| TUP | 40,218,032 | 40,218,058 | TCATGTGCTTACCCCTTTTCCTGATGC |
| TUR | 40,217,994 | 40,218,015 | GCCCATTGTTTCTGTGAGAGTC |
| TDF | 40,206,736 | 40,206,756 | GAGCATCCTAACGGTGCAAAG |
| TDP | 40,206,704 | 40,206,728 | CCCTTCAGCAAAGGTATCCAGCCCA |
| TDR | 40,206,677 | 40,206,699 | AAGCTGTCTCCTAGCACTATTGG |
PCR Primers
| Primer | Sequence |
| SU | GTCTGGAGCTGAGTTGGAGG |
| SD | AAGATTATCAGCTTTGTGGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17903A-D12 | VGB6 | C57BL/6NTac | |
| 17903A-E6 | VGB6 | C57BL/6NTac | |
