| Gene | Gstt1 |
| Chromosome | 10 |
| Deletion Start | 75,798,493 |
| Deletion End | 75,783,944 |
| Deletion Size | 14,550 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,798,155 | 75,798,176 | GTGCAGACCTAGCAACAGAAAC |
| TUP | 75,798,123 | 75,798,150 | AAGCAGTTTGGACCTAGCTACAGGGACC |
| TUR | 75,798,096 | 75,798,115 | ATGCCCTACACCCACTACTC |
| TDF | 75,784,240 | 75,784,263 | GCCGTAAATTCTGTTCCCTTCTTG |
| TDP | 75,784,214 | 75,784,238 | TATAGCCTGTAGGTGGTGGCTGCCC |
| TDR | 75,784,190 | 75,784,210 | AGCCTGGGATGCCCTTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAACTAAACCGGCCACAAG |
| SD | ATGACTTCATGGCTGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17904A-D4 | VGB6 | C57BL/6NTac | |
| 17904A-F12 | VGB6 | C57BL/6NTac | |
| 17904A-G11 | VGB6 | C57BL/6NTac | |
| 17904A-C11 | VGB6 | C57BL/6NTac | |
