| Gene | Jup |
| Chromosome | 11 |
| Deletion Start | 100,386,377 |
| Deletion End | 100,372,309 |
| Deletion Size | 14,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,385,459 | 100,385,479 | GGACAGTTGCAGGATGAGTTG |
| TUP | 100,385,430 | 100,385,455 | CTGGTGAGGGCTCTATGAACTGAGCC |
| TUR | 100,385,407 | 100,385,428 | AGGAAGGAGACCTGGGACACAG |
| TDF | 100,372,986 | 100,373,007 | ACTAGCCCACACCCACATCCAG |
| TDP | 100,372,955 | 100,372,982 | TGTCTTGCTCTAATTTGCACACGGGTAC |
| TDR | 100,372,915 | 100,372,937 | TCGGGAAGAGACATAGACACAGG |
PCR Primers
| Primer | Sequence |
| SU | CTACTGGAAATGGTGGGTGG |
| SD | TCATCATCAGAAAGGCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17908A-A10 | VGB6 | C57BL/6NTac | |
| 17908A-C6 | VGB6 | C57BL/6NTac | |
| 17908A-C9 | VGB6 | C57BL/6NTac | |
