| Gene | Clps |
| Chromosome | 17 |
| Deletion Start | 28,560,654 |
| Deletion End | 28,558,230 |
| Deletion Size | 2,425 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,560,628 | 28,560,649 | GGTCCTTGTTCTTCTGCTTGTG |
| TUP | 28,560,605 | 28,560,627 | TCCCTCCTTGCAGTGGCCTATGC |
| TUR | 28,560,556 | 28,560,577 | ACAGAGGTTCCTTACCAGGTTG |
| TDF | 28,558,328 | 28,558,346 | ATGGCATCTGCCTCGACTC |
| TDP | 28,558,304 | 28,558,327 | CAGGCGCTCCAAGCAGTGAGATCA |
| TDR | 28,558,259 | 28,558,280 | GGGACAGAGGGAGAAAGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCCATAGTCCCATGAACTGG |
| SD | TGAAATTCTGGGAGGGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17914A-B11 | VGB6 | C57BL/6NTac | |
| 17914A-A12 | VGB6 | C57BL/6NTac | |
| 17914A-D3 | VGB6 | C57BL/6NTac | |
| 17914A-D7 | VGB6 | C57BL/6NTac | |
