| Gene | Ctsb |
| Chromosome | 14 |
| Deletion Start | 63,142,355 |
| Deletion End | 63,133,344 |
| Deletion Size | 9,012 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,133,546 | 63,133,564 | AACGGGCTGTAAGGCATTC |
| TUP | 63,133,568 | 63,133,596 | TCTGCAAGTTGAAGCTCATAGACCACACC |
| TUR | 63,133,600 | 63,133,621 | ACATGGAGACTGGAGAGCAATC |
| TDF | 63,141,511 | 63,141,531 | CCGAAGCTCCCTGATAAAGAC |
| TDP | 63,141,538 | 63,141,559 | TGCTGGAGACCCAACCCAGGAC |
| TDR | 63,141,581 | 63,141,599 | GGAGGAGGGCCTCAAGATG |
PCR Primers
| Primer | Sequence |
| SU | CTTAGAATTCCCAGTACAGAG |
| SD | AGGCACTACAAACCGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17916A-B4 | VGB6 | C57BL/6NTac | |
| 17916A-B12 | VGB6 | C57BL/6NTac | |
| 17916A-C2 | VGB6 | C57BL/6NTac | |
| 17916A-H3 | VGB6 | C57BL/6NTac | |
| 17916A-G4 | VGB6 | C57BL/6NTac | |
| 17916A-E8 | VGB6 | C57BL/6NTac | |
