| Gene | Dhx58 |
| Chromosome | 11 |
| Deletion Start | 100,704,065 |
| Deletion End | 100,695,050 |
| Deletion Size | 9,016 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,703,405 | 100,703,426 | CCAGCCTCGCTTTCATTCCTAC |
| TUP | 100,703,384 | 100,703,404 | CTGCTCCCTGTCTGGGACCCA |
| TUR | 100,703,357 | 100,703,380 | TTTCCTCTTCCCTTCCCTACTTTG |
| TDF | 100,695,390 | 100,695,410 | GGGCTGGTCATCACAGAAGTC |
| TDP | 100,695,355 | 100,695,383 | AAGGATAGAAAGCCACGTGCTTCCATCTG |
| TDR | 100,695,298 | 100,695,319 | CACTGGCAAGGTCACTGATTTG |
PCR Primers
| Primer | Sequence |
| SU | GAAATAGGGAACCCAGGAGC |
| SD | TTGACTAGGAGGAGGCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17917A-G4 | VGB6 | C57BL/6NTac | |
| 17917A-D2 | VGB6 | C57BL/6NTac | |
| 17917A-D8 | VGB6 | C57BL/6NTac | |
