17918   Exoc3     Status: ES cell colonies screened / QC positives

GeneExoc3
Chromosome13
Deletion Start74,207,077
Deletion End74,172,003
Deletion Size35,075
Genome Build38
MGI IDMGI:2443972
NCBI Gene ID211446
BAC ID464P6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,206,93174,206,952CAGAAGGGAAGCTCGGAAGAAG
TUP74,206,89574,206,923TAGAGGCCAGGCTAAAGGTAGGAGACCAT
TUR74,206,84974,206,871AGTGGCGAAGAGAAACTCAAGAC
TDF74,172,18474,172,203CCAGCCGGGACATGAAACAG
TDP74,172,16174,172,183ACCATCATGGAGACGCTGGAGCA
TDR74,172,09674,172,115TCAGACTGGGCACAACAATC

PCR Primers

PrimerSequence
SUGGCTGCTGCTGAAGTGTAGG
SDACGTGGACATCCTCTATCGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR104 bp++-
TDFTDR108 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17918A-G12VGB6C57BL/6NTac
17918A-F1VGB6C57BL/6NTac
17918A-A12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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