| Gene | Fkbp10 |
| Chromosome | 11 |
| Deletion Start | 100,424,093 |
| Deletion End | 100,415,787 |
| Deletion Size | 8,307 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,416,302 | 100,416,319 | GTACCCTGGCACGCAATC |
| TUP | 100,416,324 | 100,416,351 | AGGGCAAAGAGACTGTGCCTACTACACG |
| TUR | 100,416,360 | 100,416,382 | CAGACCATCCATCCACTCACTTC |
| TDF | 100,423,602 | 100,423,623 | GGGTCAGGAACTCAGTCACAAC |
| TDP | 100,423,624 | 100,423,645 | CCCAACCCAGAAGCCAGCATGC |
| TDR | 100,423,654 | 100,423,674 | GAGCAAAGTAGGCAGCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTACAGCCTCACCTGCACC |
| SD | AGAGCTGAGGCAGAAGAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17920A-A3 | VGB6 | C57BL/6NTac | |
| 17920A-B1 | VGB6 | C57BL/6NTac | |
| 17920A-D6 | VGB6 | C57BL/6NTac | |
| 17920A-G12 | VGB6 | C57BL/6NTac | |
| 17920A-B11 | VGB6 | C57BL/6NTac | |
| 17920A-B6 | VGB6 | C57BL/6NTac | |
