| Gene | Pik3r5 |
| Chromosome | 11 |
| Deletion Start | 68,496,323 |
| Deletion End | 68,473,811 |
| Deletion Size | 22,513 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,473,933 | 68,473,954 | CGTTCCAGAGCCTCAGATGAAG |
| TUP | 68,473,963 | 68,473,988 | TGGAACCTCTGGTCTTGCTTCTTGGA |
| TUR | 68,474,012 | 68,474,034 | TGAGCTGAGACCAGCATAAGAAG |
| TDF | 68,495,727 | 68,495,744 | TAAGAGGCTGCGGAGGAG |
| TDP | 68,495,746 | 68,495,773 | CAGGGCCTGGCATAGTTCTCATGATTCC |
| TDR | 68,495,806 | 68,495,825 | GGGAGAGTTTGAGACAGGAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGGAGACACAGGTCTGG |
| SD | GGAAAGAAATGTCTCACCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17921A-D1 | VGB6 | C57BL/6NTac | |
| 17921A-F8 | VGB6 | C57BL/6NTac | |
