| Gene | Set |
| Chromosome | 2 |
| Deletion Start | 30,071,014 |
| Deletion End | 30,062,175 |
| Deletion Size | 8,840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,062,589 | 30,062,610 | CCACGAGTCCTGGAGATTCAAG |
| TUP | 30,062,613 | 30,062,637 | CGCTCTGATCGTCTCCCTGTTGCTG |
| TUR | 30,062,662 | 30,062,683 | ACACGAGGTGGCAGTAATTCAG |
| TDF | 30,070,813 | 30,070,833 | TCCCTCCACATCAGTGCTAAG |
| TDP | 30,070,838 | 30,070,865 | TGAAGATTCCAGTTTAGGCTGGGAGAGC |
| TDR | 30,070,909 | 30,070,930 | GCCTTCGTCCTCCTGAAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGGTGAAGGGAAAGAGGAAG |
| SD | GACACAGTGTAATCTCTGAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17924A-A11 | VGB6 | C57BL/6NTac | |
| 17924A-B6 | VGB6 | C57BL/6NTac | |
| 17924A-G12 | VGB6 | C57BL/6NTac | |
| 17924A-G9 | VGB6 | C57BL/6NTac | |
| 17924A-H7 | VGB6 | C57BL/6NTac | |
