17925   Tmsb15a     Status: ES cell colonies screened / QC positives

GeneTmsb15a
ChromosomeX
Deletion Start135,719,762
Deletion End135,718,712
Deletion Size1,051
Genome Build38
MGI IDMGI:1925728
NCBI Gene ID78478
BAC ID267G14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,719,350135,719,371CACATCTTCCAGGATGCAAACC
TUP135,719,314135,719,338AGCTTTATCCATAGAAAACTATGCA
TUR135,719,240135,719,260AGCCTGACCACACCTAAGCAC
TDF135,719,210135,719,234GACTCAAGGTTTGCTCTTCAGAAAG
TDP135,719,185135,719,209ACAGCCTCCTGGAGGAGGTGAAAGT
TDR135,719,153135,719,173GGACATAAACTGCCTCTCAGC

PCR Primers

PrimerSequence
SUGCTCCTGGCCTCTAACTGTG
SDCTTAACTGGGTACTTATTAACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR132 bp++-
TDFTDR82 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17925A-D1VGB6C57BL/6NTac
17925A-H6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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