| Gene | Zfp523 |
| Chromosome | 17 |
| Deletion Start | 28,205,241 |
| Deletion End | 28,189,883 |
| Deletion Size | 15,359 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,190,470 | 28,190,490 | TCTGTCAGAGGACCTGAGTTG |
| TUP | 28,190,496 | 28,190,519 | CCAGCACCTATGATGGGCTGCTCC |
| TUR | 28,190,523 | 28,190,543 | TCAGACGAGGGTGTTGGATTC |
| TDF | 28,204,354 | 28,204,376 | TGCAATCACAATCACCTCCACAG |
| TDP | 28,204,377 | 28,204,398 | CGCAGTGACCTTGTCCGTCCCG |
| TDR | 28,204,431 | 28,204,451 | GAGACACAGAAGCAGAAGACC |
PCR Primers
| Primer | Sequence |
| SU | TCTTGACCACCATGCTTCTG |
| SD | GAGACGGTTATTGCTGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17927A-A5 | VGB6 | C57BL/6NTac | |
| 17927A-A11 | VGB6 | C57BL/6NTac | |
| 17927A-G10 | VGB6 | C57BL/6NTac | |
| 17927A-C6 | VGB6 | C57BL/6NTac | |
| 17927A-G7 | VGB6 | C57BL/6NTac | |
