| Gene | Zcwpw1 |
| Chromosome | 5 |
| Deletion Start | 137,822,560 |
| Deletion End | 137,795,521 |
| Deletion Size | 27,040 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,795,667 | 137,795,685 | TGGTGCCAGTTGCATTGAG |
| TUP | 137,795,686 | 137,795,714 | TGAAGTAGGTATGGGTTAGAAGCAGCCCT |
| TUR | 137,795,715 | 137,795,738 | CTGAGCTATCTCTTTCGTCCCATC |
| TDF | 137,821,604 | 137,821,628 | GCTGGATAATGGTGTTGGATTTCTC |
| TDP | 137,821,630 | 137,821,652 | TAGCGCCTGCGATCTGTCTACGC |
| TDR | 137,821,661 | 137,821,682 | ATACCTGGCCCTGTGGGTAGTG |
PCR Primers
| Primer | Sequence |
| SU | GGCCTTGACAACTTACGGAG |
| SD | CTGACTCCATGACATGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17928B-A3 | VGB6 | C57BL/6NTac | |
