| Gene | Cdkn2c |
| Chromosome | 4 |
| Deletion Start | 109,665,143 |
| Deletion End | 109,661,169 |
| Deletion Size | 3,975 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,664,528 | 109,664,550 | ACGACCTTTCCTCGCTTGGTATG |
| TUP | 109,664,504 | 109,664,525 | ATAGCCTGGGCACCCGGACAGG |
| TUR | 109,664,453 | 109,664,475 | TCACTCCTGAATGGGTGGATTAG |
| TDF | 109,661,500 | 109,661,521 | AGAGCAGGTTTCCTGGACACTG |
| TDP | 109,661,475 | 109,661,497 | CAGGCTTTGCTGGAGTTCCAGGC |
| TDR | 109,661,403 | 109,661,423 | ACAGGGAGGTGGCCTTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GTCTCCGCAACATCTTCCTC |
| SD | ACACCAGGCTTCCAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17929A-H6 | VGB6 | C57BL/6NTac | |
| 17929A-C4 | VGB6 | C57BL/6NTac | |
| 17929A-A9 | VGB6 | C57BL/6NTac | |
| 17929A-D1 | VGB6 | C57BL/6NTac | |
