| Gene | Lipt1 |
| Chromosome | 1 |
| Deletion Start | 37,876,085 |
| Deletion End | 37,874,868 |
| Deletion Size | 1,218 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,875,389 | 37,875,409 | CCACCACTGCACCTTGCTATG |
| TUP | 37,875,410 | 37,875,435 | CAGCACCAACCGGACAGCTCTGTCTT |
| TUR | 37,875,441 | 37,875,459 | CGCAGTACGGGCTCTTCAG |
| TDF | 37,875,683 | 37,875,703 | CGGCAGGACTCCCAAGTTTAC |
| TDP | 37,875,704 | 37,875,727 | CGTGGACACCACGTTTCATGTGCC |
| TDR | 37,875,728 | 37,875,747 | CCAAATGCGCCTGCTCATAC |
PCR Primers
| Primer | Sequence |
| SU | TACTCCATATCATTGACTTCAG |
| SD | CAATGGTACAAGCAGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17932A-A6 | VGB6 | C57BL/6NTac | |
| 17932A-D11 | VGB6 | C57BL/6NTac | |
| 17932A-E1 | VGB6 | C57BL/6NTac | |
| 17932A-E6 | VGB6 | C57BL/6NTac | |
| 17932A-F4 | VGB6 | C57BL/6NTac | |
| 17932A-G1 | VGB6 | C57BL/6NTac | |
