| Gene | Mitd1 |
| Chromosome | 1 |
| Deletion Start | 37,890,307 |
| Deletion End | 37,879,253 |
| Deletion Size | 11,055 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,889,681 | 37,889,699 | TGCACCAACCAGTTGTCAG |
| TUP | 37,889,654 | 37,889,679 | CTCTGCAGTCACTACCCGGTACTGGT |
| TUR | 37,889,623 | 37,889,645 | TGTAGTCCACATGGTCAGAGAAG |
| TDF | 37,879,548 | 37,879,569 | GAAACCACAGGTAGGCTATGAC |
| TDP | 37,879,520 | 37,879,544 | ATGATCCCGTGTGAGCACTGCTTCC |
| TDR | 37,879,461 | 37,879,484 | CAGGAGCCCATCAGAACAAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAAGAAATAGGTCTGCGGG |
| SD | GCTGAACAGCATATTTACAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17933A-E4 | VGB6 | C57BL/6NTac | |
| 17933A-F11 | VGB6 | C57BL/6NTac | |
| 17933A-B8 | VGB6 | C57BL/6NTac | |
| 17933A-H11 | VGB6 | C57BL/6NTac | |
| 17933A-D3 | VGB6 | C57BL/6NTac | |
| 17933A-C7 | VGB6 | C57BL/6NTac | |
