| Gene | Snupn |
| Chromosome | 9 |
| Deletion Start | 56,983,068 |
| Deletion End | 56,957,022 |
| Deletion Size | 26,047 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,957,770 | 56,957,788 | GCTGTGCCACTTGCATCTC |
| TUP | 56,957,790 | 56,957,818 | TCTGGACTTTGTGAGGTGCTATGTCAGTA |
| TUR | 56,957,844 | 56,957,866 | GATCAGCAGACAACTCAGAACTC |
| TDF | 56,982,405 | 56,982,427 | GCATGGTTGCACCTCCTTTAATC |
| TDP | 56,982,428 | 56,982,450 | TCAGCACTCAGAAGGTGGAGGCT |
| TDR | 56,982,473 | 56,982,496 | TGTCCTGCTACATAGACCAAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTTTTTTTTAGTGCTTTGTTTTG |
| SD | GTGGGCCAGGATCTCAATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17934A-H10 | VGB6 | C57BL/6NTac | |
| 17934A-F10 | VGB6 | C57BL/6NTac | |
| 17934A-E11 | VGB6 | C57BL/6NTac | |
| 17934A-C9 | VGB6 | C57BL/6NTac | |
