| Gene | Whsc1l1 |
| Chromosome | 8 |
| Deletion Start | 25,666,151 |
| Deletion End | 25,640,624 |
| Deletion Size | 25,528 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,640,661 | 25,640,684 | ACACAATTCAGCAACCACCTCAAC |
| TUP | 25,640,687 | 25,640,711 | ATTGACTCGGCCAACATCCGCCAGG |
| TUR | 25,640,741 | 25,640,759 | TCGGGCCACCATCTTCAAC |
| TDF | 25,665,936 | 25,665,957 | GTTTGAGCAAATTACGGTCACG |
| TDP | 25,665,958 | 25,665,980 | TTAAGCCATCTGTATAAGCTCAT |
| TDR | 25,665,981 | 25,666,005 | GACAAGTTAGTAGGCACATAACAGC |
PCR Primers
| Primer | Sequence |
| SU | TGCATCCTAAAGTTGCTATGG |
| SD | TTAGCATACAGAGCAAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17935A-D1 | VGB6 | C57BL/6NTac | |
| 17935A-F4 | VGB6 | C57BL/6NTac | |
| 17935A-H3 | VGB6 | C57BL/6NTac | |
