| Gene | Lmnb1 |
| Chromosome | 18 |
| Deletion Start | 56,740,034 |
| Deletion End | 56,708,116 |
| Deletion Size | 31,919 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,708,430 | 56,708,448 | CGAGCTGGGCAAGTTCAAG |
| TUP | 56,708,453 | 56,708,473 | AGCACGACCAGCTGCTGCTCA |
| TUR | 56,708,489 | 56,708,508 | AGCCTCTCGGCGTGACAATC |
| TDF | 56,739,720 | 56,739,744 | GGGCTGTATCTACTTTCTGCTTCAG |
| TDP | 56,739,748 | 56,739,775 | TGGTTTGAAGTAGCTGCCTTGTGCTCAT |
| TDR | 56,739,797 | 56,739,817 | CAGGGTCAAAGGAGCACATTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGGAAACAAAGTGCTGC |
| SD | CAGGATCGACAGGTCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17937A-H7 | VGB6 | C57BL/6NTac | |
| 17937B-B4 | VGB6 | C57BL/6NTac | |
| 17937B-B12 | VGB6 | C57BL/6NTac | |
| 17937B-D5 | VGB6 | C57BL/6NTac | |
