| Gene | Arl13b |
| Chromosome | 16 |
| Deletion Start | 62,846,740 |
| Deletion End | 62,827,169 |
| Deletion Size | 19,572 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,845,939 | 62,845,963 | TGTCAAGAAACATATACAGGCACTG |
| TUP | 62,845,909 | 62,845,938 | TGCTAGGGTGGAGTCAGGGAAATTGATGTT |
| TUR | 62,845,887 | 62,845,907 | GTTTGGGCTTCTGGGTTTCAG |
| TDF | 62,827,981 | 62,828,000 | TCAGGAGGCATGTGGATCTC |
| TDP | 62,827,958 | 62,827,979 | TACTACCAGCCCGGACCACAGA |
| TDR | 62,827,936 | 62,827,956 | CACTGGCTGTCGAGGAACTTG |
PCR Primers
| Primer | Sequence |
| SU | TCTGATTGGAGAAGCGTGTG |
| SD | AGAACAGGGCTGAAAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17941A-C2 | VGB6 | C57BL/6NTac | |
| 17941A-B12 | VGB6 | C57BL/6NTac | |
| 17941A-G8 | VGB6 | C57BL/6NTac | |
| 17941A-H6 | VGB6 | C57BL/6NTac | |
