| Gene | Cln3 |
| Chromosome | 7 |
| Deletion Start | 126,583,042 |
| Deletion End | 126,572,222 |
| Deletion Size | 10,821 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,582,999 | 126,583,019 | GCGCCTTGAGGATTCTGAGAG |
| TUP | 126,582,974 | 126,582,998 | TGAGTGCTCTCATCGGTTGCCTTGG |
| TUR | 126,582,955 | 126,582,973 | CGGGCAGAGGTGGTCATTC |
| TDF | 126,572,433 | 126,572,452 | AGGGCTGTGGGTGGCCTTAG |
| TDP | 126,572,403 | 126,572,429 | CCTTGTGGATGGAAGCCCTAACCCTTG |
| TDR | 126,572,370 | 126,572,389 | CGGTGCTTGTCACTGGTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTCGTCTACCTTGCGGAGAG |
| SD | ATCTTTCACAGTGGGCTTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17942A-G2 | VGB6 | C57BL/6NTac | |
| 17942A-D2 | VGB6 | C57BL/6NTac | |
| 17942A-G1 | VGB6 | C57BL/6NTac | |
| 17942A-F1 | VGB6 | C57BL/6NTac | |
