| Gene | Dtwd1 |
| Chromosome | 2 |
| Deletion Start | 126,165,006 |
| Deletion End | 126,154,600 |
| Deletion Size | 10,407 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,154,742 | 126,154,763 | AGGCTCAACAAAGTGGGAGGTC |
| TUP | 126,154,768 | 126,154,789 | TGTCGCCAATGTGGTGGCTCAA |
| TUR | 126,154,794 | 126,154,816 | CACAGCATGTGTAGCAGTAGAAC |
| TDF | 126,164,914 | 126,164,936 | CCGCTTGATAAAGAATGCCAGAG |
| TDP | 126,164,941 | 126,164,963 | TGGAGAGAAGGCTAAACCGCAGC |
| TDR | 126,164,971 | 126,164,988 | GCGGGTGTGCAGAACTAC |
PCR Primers
| Primer | Sequence |
| SU | TGGGTACTATCAGAATGGATTGTC |
| SD | TAAGTCGAAAGGTAAGGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17943A-H7 | VGB6 | C57BL/6NTac | |
