| Gene | Ftsj1 |
| Chromosome | X |
| Deletion Start | 8,250,997 |
| Deletion End | 8,245,075 |
| Deletion Size | 5,923 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,250,462 | 8,250,481 | AGGGTTCTGGTCAGGTGGTG |
| TUP | 8,250,435 | 8,250,460 | CCGTGGACTTACAGGCTATGGCTCCA |
| TUR | 8,250,404 | 8,250,425 | TGTCGCCCTGTATCTGTATCAC |
| TDF | 8,245,289 | 8,245,310 | GCTATCCATACCCTGCTAGACC |
| TDP | 8,245,253 | 8,245,280 | TGGATATGTACCTCTGAGCCCACCATGA |
| TDR | 8,245,224 | 8,245,242 | AGGACAGAAACGGGCACAC |
PCR Primers
| Primer | Sequence |
| SU | TGTGTCCGTGACAGTAGTTGG |
| SD | GTGCTTGTGAATGTAGGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17944A-G8 | VGB6 | C57BL/6NTac | |
| 17944A-H1 | VGB6 | C57BL/6NTac | |
