| Gene | Hmgb1 |
| Chromosome | 5 |
| Deletion Start | 149,050,734 |
| Deletion End | 149,048,709 |
| Deletion Size | 2,026 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 149,050,206 | 149,050,227 | CGAGTGCCTAGTGTTTGGTTTC |
| TUP | 149,050,176 | 149,050,203 | AGCTGAGAATGGCTTGGGTCGTGTATTT |
| TUR | 149,050,098 | 149,050,119 | ACTGTGCAAAGGAGTGAGTTTC |
| TDF | 149,049,203 | 149,049,225 | AAACTCTGGCTGTGAACCTGTAG |
| TDP | 149,049,175 | 149,049,200 | TGCTGTTTTGTGTTCAGAAGGTGGCA |
| TDR | 149,049,083 | 149,049,105 | TGTGTTGTTATGCCTCACTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTTGAGTGTACCTGTGCC |
| SD | GCTGCATCAGAGACAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 143 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17946B-C6 | VGB6 | C57BL/6NTac | |
