| Gene | Pkn3 |
| Chromosome | 2 |
| Deletion Start | 30,090,767 |
| Deletion End | 30,078,779 |
| Deletion Size | 11,989 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,079,415 | 30,079,436 | GAAAGGGAGACAGGAGCTAGAC |
| TUP | 30,079,438 | 30,079,462 | CTGCAGGGTTCTGAGACACTCTGGG |
| TUR | 30,079,514 | 30,079,532 | TGCAAAGGGCAGAGAAGTC |
| TDF | 30,090,115 | 30,090,135 | CAGTGCTGATGTCCCATGTCC |
| TDP | 30,090,136 | 30,090,161 | CCACTTTCTGTCAGTGCAAGGGCTCG |
| TDR | 30,090,205 | 30,090,224 | GCAGGGTACACCCAAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGAAAGGCTGGATCTCAC |
| SD | GTCTTCCTAGGGCTTCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17947A-C7 | VGB6 | C57BL/6NTac | |
| 17947A-B7 | VGB6 | C57BL/6NTac | |
| 17947A-D4 | VGB6 | C57BL/6NTac | |
| 17947A-G9 | VGB6 | C57BL/6NTac | |
| 17947A-C12 | VGB6 | C57BL/6NTac | |
