| Gene | Rsl24d1 |
| Chromosome | 9 |
| Deletion Start | 73,122,432 |
| Deletion End | 73,113,506 |
| Deletion Size | 8,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,113,718 | 73,113,737 | TGCTGTCAGACGCTCATCTC |
| TUP | 73,113,738 | 73,113,757 | ATCCGCAGGCCACCCGGCTT |
| TUR | 73,113,848 | 73,113,866 | AAGGCTCAGGCACCATGTC |
| TDF | 73,121,880 | 73,121,900 | CACGATGCAGAGCCCAGAAAG |
| TDP | 73,121,901 | 73,121,924 | AGCTGAGAGCCTGCTTCCTTTGGC |
| TDR | 73,121,952 | 73,121,971 | CAGAGCCACATGCCCTACAC |
PCR Primers
| Primer | Sequence |
| SU | TGCAGTCTATAGCCTCTGCG |
| SD | CTGTGCTGTGTTGTGTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 149 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17949A-A1 | VGB6 | C57BL/6NTac | |
| 17949A-C5 | VGB6 | C57BL/6NTac | |
| 17949A-F12 | VGB6 | C57BL/6NTac | |
