| Gene | Sbds |
| Chromosome | 5 |
| Deletion Start | 130,255,334 |
| Deletion End | 130,246,295 |
| Deletion Size | 9,040 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,254,516 | 130,254,538 | CAGTTTCCCAAAGTGCGTAGTAG |
| TUP | 130,254,489 | 130,254,513 | TCTTCGCTACTGCCATCTAGGGACA |
| TUR | 130,254,459 | 130,254,481 | GGAGTGGTGTTTCTTCTGTCCTG |
| TDF | 130,246,521 | 130,246,541 | CTGCCCTGAGCATTCAAACTG |
| TDP | 130,246,491 | 130,246,518 | CTTATTCTGTGCCGATGCCCATCTTCCC |
| TDR | 130,246,444 | 130,246,461 | TGGGTCGATGAGGCACAC |
PCR Primers
| Primer | Sequence |
| SU | AACTGGCGCCTGTAAATGAC |
| SD | GGGACCCAGGAACTCTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17950A-D2 | VGB6 | C57BL/6NTac | |
| 17950A-E7 | VGB6 | C57BL/6NTac | |
| 17950A-F8 | VGB6 | C57BL/6NTac | |
| 17950A-E12 | VGB6 | C57BL/6NTac | |
| 17950A-F1 | VGB6 | C57BL/6NTac | |
| 17950A-B10 | VGB6 | C57BL/6NTac | |
