| Gene | Sfxn1 |
| Chromosome | 13 |
| Deletion Start | 54,106,661 |
| Deletion End | 54,085,463 |
| Deletion Size | 21,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,085,473 | 54,085,495 | TGCCACCCAACATTAACATCAAG |
| TUP | 54,085,497 | 54,085,519 | AGCCTCGATGGGACCAGAGCACG |
| TUR | 54,085,540 | 54,085,562 | TGGGATCAGTAACCGTGAAGAAG |
| TDF | 54,106,412 | 54,106,431 | GCCCTGGGTCTCTGAAACTG |
| TDP | 54,106,434 | 54,106,458 | CGCTTTCCCTGTCCTTCCTAGCTCA |
| TDR | 54,106,486 | 54,106,509 | GAACTGGGACAAGGTAGAAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAGTGTGTGCACTGTGTG |
| SD | AAATGGAATCTGGTGGTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17951A-B8 | VGB6 | C57BL/6NTac | |
| 17951A-C10 | VGB6 | C57BL/6NTac | |
| 17951A-C11 | VGB6 | C57BL/6NTac | |
| 17951A-D9 | VGB6 | C57BL/6NTac | |
| 17951A-E12 | VGB6 | C57BL/6NTac | |
| 17951A-H2 | VGB6 | C57BL/6NTac | |
