| Gene | Smad4 |
| Chromosome | 18 |
| Deletion Start | 73,677,908 |
| Deletion End | 73,640,240 |
| Deletion Size | 37,669 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,677,208 | 73,677,230 | CATTCATGTCATGGCTCACACTC |
| TUP | 73,677,180 | 73,677,201 | CACCTGCCTACCCTTGGCGGTA |
| TUR | 73,677,099 | 73,677,121 | CCACTGCTTGTTTCATACCGATG |
| TDF | 73,640,355 | 73,640,376 | CCAGGCAGAGCATCAAGGAAAC |
| TDP | 73,640,328 | 73,640,354 | CCCGTGCTGGATTGAGATTCACCTTCA |
| TDR | 73,640,286 | 73,640,305 | GGCATGGTGTGCAGGACTTC |
PCR Primers
| Primer | Sequence |
| SU | CTATGGGCATTGAGTCTTTTGAC |
| SD | AGCAGCTCTGACTCTCCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17952A-A8 | VGB6 | C57BL/6NTac | |
| 17952A-G6 | VGB6 | C57BL/6NTac | |
| 17952A-B8 | VGB6 | C57BL/6NTac | |
| 17952A-B9 | VGB6 | C57BL/6NTac | |
