| Gene | Tbcb |
| Chromosome | 7 |
| Deletion Start | 30,231,690 |
| Deletion End | 30,224,228 |
| Deletion Size | 7,463 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,231,632 | 30,231,652 | CATCAGCAGCTCCCTCAACAG |
| TUP | 30,231,605 | 30,231,629 | TCCGTTCGGAGAAGCGATACAGTCG |
| TUR | 30,231,571 | 30,231,588 | CGGCCACACCTTGAACTC |
| TDF | 30,224,298 | 30,224,317 | GGCCAAGTATGGTGCCTTTG |
| TDP | 30,224,272 | 30,224,296 | AAAGCCATCAGCTGTCACCGTAGGA |
| TDR | 30,224,236 | 30,224,256 | CATCTCGTCCAACCCGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | GCCATTCTTCCCGGCTTC |
| SD | CCACTTCCCAGTGTTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17953A-H6 | VGB6 | C57BL/6NTac | |
| 17953A-B6 | VGB6 | C57BL/6NTac | |
| 17953A-D3 | VGB6 | C57BL/6NTac | |
| 17953A-D5 | VGB6 | C57BL/6NTac | |
