| Gene | Ppp2r4 |
| Chromosome | 2 |
| Deletion Start | 30,446,459 |
| Deletion End | 30,416,305 |
| Deletion Size | 30,155 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,416,793 | 30,416,812 | GTGGCATCGTCGTCTGTAAC |
| TUP | 30,416,816 | 30,416,843 | TATTTACACTGTGGCCTCGTTGTGGTCC |
| TUR | 30,416,861 | 30,416,880 | GCATCTGGCCTCTGTCTCTG |
| TDF | 30,445,680 | 30,445,700 | CACTTCTGAAGCTGCTGTCTG |
| TDP | 30,445,710 | 30,445,732 | AAGGTGAGCCACCAGGTTCGTCC |
| TDR | 30,445,761 | 30,445,783 | GAGGTGACCTGTAGGCTATTGAG |
PCR Primers
| Primer | Sequence |
| SU | TAGTGCGCATGCTCCTCG |
| SD | TCACCCTTCAGCTCCATAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17955A-B9 | VGB6 | C57BL/6NTac | |
| 17955B-D12 | VGB6 | C57BL/6NTac | |
| 17955B-F5 | VGB6 | C57BL/6NTac | |
